Originally Posted by luisillo
Try running it in a less concentrated gel (for instance 1%) and for a bit more of time. Raising the voltage a little wouldn't harm. If it still doesn't separate then call the supplier for troubleshooting.
I have contacted the supplier, they said the dna ladder i used contain Orange Dye which is not compatible with the GelRed I use to stain the agarose.
but I will try and run it again with lesser agarose concentration and see if there are any changes.
Thanks for replying.