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PCR product can not migrate into gel

PCR product can not migrate into gel - Agarose Gel Electrophoresis Forum

PCR product can not migrate into gel - Agarose Gel Electrophoresis Forum. A forum to discuss electrophoresis staining, troubleshooting, and development of films for DNA and RNA gels.


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Old 08-16-2011, 04:33 PM
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Default Re: PCR product can not migrate into gel

I must ask you first. Is this is first time are you doing this PCR, did you ever got PCR product for this PCR reaction.

It look like your PCR reaction didnot worked. what ever you see may be only salts stacked in the well.
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Old 08-19-2011, 06:19 AM
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Default Re: PCR product can not migrate into gel

Quote:
Originally Posted by obama View Post
I must ask you first. Is this is first time are you doing this PCR, did you ever got PCR product for this PCR reaction.

It look like your PCR reaction didnot worked. what ever you see may be only salts stacked in the well.
Thanks. I foget how many times I did this PCR reaction. It was performed well before it happened to that problem while I shoewd to you in the forum.
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Old 03-14-2012, 02:18 PM
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Default Mitochondrial Genome Extraction

Dear
Can anybody suggest a methodology to isolate and sequence mitochondrial genome of an organism
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Old 10-22-2012, 09:52 PM
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Default Re: PCR product can not migrate into gel

It looks like the template concentration is too high. I would dilute template and redo pcr reaction (you shouldn't be able to see your template on the pcr reaction gel).
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