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Contamination disappears!

Contamination disappears! - Agarose Gel Electrophoresis Forum

Contamination disappears! - Agarose Gel Electrophoresis Forum. A forum to discuss electrophoresis staining, troubleshooting, and development of films for DNA and RNA gels.


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Old 06-24-2011, 03:59 PM
Pipette Filler
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Angry Contamination disappears!



I have a parental plasmid at 6.3kb, and this contamination just started appearing at 3.2kb. Everytime I run the gel out longer so I can separate bands and excise, gel purify, and retransform to purify my parental plasmid, the contamination disappears. I've tried variations in agarose %, time, and voltage. The variable that affects the appearance of the contamination is time, when I run it for awhile to separate bands it disappears. I ran the gel for a short time and carefully excised the band and ran a gel purification, but my spec reading was almost 0. What contamination could be time dependent, but continue to show up after quick change mutagenesis, minipreps, maxipreps, and many re-transformations?
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