Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Molecular Biology Techniques > Agarose Gel Electrophoresis Forum
Register Search Today's Posts Mark Forums Read

Agarose Gel Electrophoresis Forum Agarose Gel Electrophoresis Forum. A forum to discuss electrophoresis staining, troubleshooting, and development of films for DNA and RNA gels.


2DE vertical streaking

2DE vertical streaking - Agarose Gel Electrophoresis Forum

2DE vertical streaking - Agarose Gel Electrophoresis Forum. A forum to discuss electrophoresis staining, troubleshooting, and development of films for DNA and RNA gels.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 04-07-2011, 04:20 PM
Pipette Filler
Points: 49, Level: 1 Points: 49, Level: 1 Points: 49, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Apr 2011
Posts: 3
Thanks: 1
Thanked 1 Time in 1 Post
Default 2DE vertical streaking



Can anyone help to troubleshoot my problem? Thanks.

Attached is my 12% Acrylamide gel.
Attached Images
File Type: jpg sleeve 23 R5 (2).jpg (19.4 KB, 11 views)
Reply With Quote
  #2  
Old 08-20-2011, 04:01 AM
Volunteer
Points: 231, Level: 4 Points: 231, Level: 4 Points: 231, Level: 4
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Aug 2011
Location: Malaysia
Posts: 36
Thanks: 0
Thanked 14 Times in 13 Posts
Default Re: 2DE vertical streaking

Hi, many possible reasons causing vertical streaking of gel. 1. Insufficient equilibration; 2. uneven polymerization of gel; 3. SDS-PAGE solution are not fresh; 4. Insufficient SDS in SDS electrophoresis buffer; 5. Fast migration of proteins from strip. Perhaps you can try preparing new solutions freshly for SDS-PAGE preparation and try running the gels at lower voltage. Good luck
Reply With Quote
The Following User Says Thank You to JennYeap For This Useful Post:
ywei (08-20-2011)
Reply

Tags
2de , streaking , vertical


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Gel Streaking Lisa Agarose Gel Electrophoresis Forum 3 08-03-2011 02:42 PM
Vertical vs. lateral resolution Sayeh Electron Microscopy Forum 1 10-29-2009 04:20 PM
Streaking out on agar plates CRS Molecular Biology Techniques 2 02-25-2009 02:30 AM
Troubleshooting Streaking of 2-D Gels kiki06 2-D Gel Electrophoresis Forum 2 07-03-2008 03:56 PM
horizontal streaking tina Proteomics Forum 6 11-22-2007 12:59 PM


All times are GMT. The time now is 07:33 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.12805 seconds with 17 queries