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Help! Restriction Enzyme question???

Help! Restriction Enzyme question??? - Agarose Gel Electrophoresis Forum

Help! Restriction Enzyme question??? - Agarose Gel Electrophoresis Forum. A forum to discuss electrophoresis staining, troubleshooting, and development of films for DNA and RNA gels.


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Old 03-12-2011, 02:52 PM
Pipette Filler
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Unhappy Help with an Agarose gel electrophoresis question???



I have been given the following question and am unsure about how to answer it...

Bacteriophage DNA is 48000 base pairs long. Hae lll cuts it into 206 pieces - close to what you would expect. Hind lll cuts it in 7 places, whilst another 6-cutter Pst l cuts it into 28 pieces. Why the difference?

If anyone could give me some ideas on how to answer this question it would be great.Thankyou.

Last edited by Emmalouise; 03-12-2011 at 03:17 PM.
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Old 03-13-2011, 12:31 AM
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Default Re: Help! Restriction Enzyme question???

I may not be right, but I had a similar question and here is what I came up with:

Each restriction enzyme cuts DNA at specific location as the various enzymes recognise specific sites on the DNA. Therefore some will cut more or less than others depending where in the sequence the enzyme will bind and cut. I wrote more but I am not sure about it...

If you are in my class you probably have questions prior to this one that will help you make sense of it!
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