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| Agarose Gel Electrophoresis Forum Agarose Gel Electrophoresis Forum. A forum to discuss electrophoresis staining, troubleshooting, and development of films for DNA and RNA gels. |
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#1
02-17-2011, 10:45 AM
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 DNA BANDS Not Showing up in Agarose Gel question? Hi guys, I wanted to know what are the possible reasons for DNA bands not showing up on the gel after electrophoresis. Thanks Last edited by admin; 02-17-2011 at 11:26 PM.  |
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#2
02-17-2011, 11:25 PM
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 Re: Agarose gel question Few reasons: 1) they have run off the gel or 1B) the DNA did not go into the gel (was lost in transfer - loading or similar) 2) there was too much degradation of the DNA (see 3) 3) there was too little DNA to be detected 4) your detection method did not work (ethidium bromide, or SYBR or similar not working or not present) 5) UV light or photo was not working just a few reasons |
| The Following User Says Thank You to admin For This Useful Post: | ||
grandhotel (02-18-2011)
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#3
02-18-2011, 08:05 AM
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| Re: DNA BANDS Not Showing up in Agarose Gel question? Thank you very much!  |
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| agarose , bands , dna , gel , question , showing |
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