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| Agarose Gel Electrophoresis Forum Agarose Gel Electrophoresis Forum. A forum to discuss electrophoresis staining, troubleshooting, and development of films for DNA and RNA gels. |
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#1
12-22-2010, 07:41 AM
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 agarose smears I do rt-pcr (semi-nested), my problem is when I run the gel (2% agarose; 100V; 1hr) the ladder comes out nicely seperated but my samples only show smears. Have tried DNA clean up but the problem still persists. Pliz help!!  |
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#2
12-22-2010, 12:36 PM
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| Re: agarose smears Can you post your agarose gel picture? Seems like your DNA is degraded on isolation. see these threads: DNAse Inhibition for Perfect DNA Isolations my DNA is degrading.. |
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