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Agarose Gel Electrophoresis Forum Agarose Gel Electrophoresis Forum. A forum to discuss electrophoresis staining, troubleshooting, and development of films for DNA and RNA gels.


agarose smears

agarose smears - Agarose Gel Electrophoresis Forum

agarose smears - Agarose Gel Electrophoresis Forum. A forum to discuss electrophoresis staining, troubleshooting, and development of films for DNA and RNA gels.


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Old 12-22-2010, 08:41 AM
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Default agarose smears



I do rt-pcr (semi-nested), my problem is when I run the gel (2% agarose; 100V; 1hr) the ladder comes out nicely seperated but my samples only show smears. Have tried DNA clean up but the problem still persists.

Pliz help!!
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Old 12-22-2010, 01:36 PM
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Default Re: agarose smears

Can you post your agarose gel picture?

Seems like your DNA is degraded on isolation.

see these threads:

DNAse Inhibition for Perfect DNA Isolations

my DNA is degrading..
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