- - agarose smears
|timsibanda ||12-22-2010 08:41 AM |
I do rt-pcr (semi-nested), my problem is when I run the gel (2% agarose; 100V; 1hr) the ladder comes out nicely seperated but my samples only show smears. Have tried DNA clean up but the problem still persists.
|admin ||12-22-2010 01:36 PM |
Re: agarose smears
Can you post your agarose gel picture?
Seems like your DNA is degraded on isolation.
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