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-   -   agarose smears (http://www.molecularstation.com/forum/agarose-gel-electrophoresis-forum/74717-agarose-smears.html)

timsibanda 12-22-2010 08:41 AM

agarose smears
 
I do rt-pcr (semi-nested), my problem is when I run the gel (2% agarose; 100V; 1hr) the ladder comes out nicely seperated but my samples only show smears. Have tried DNA clean up but the problem still persists.

Pliz help!!

admin 12-22-2010 01:36 PM

Re: agarose smears
 
Can you post your agarose gel picture?

Seems like your DNA is degraded on isolation.

see these threads:

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