Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Molecular Biology Techniques > Agarose Gel Electrophoresis Forum
Register Search Today's Posts Mark Forums Read

Agarose Gel Electrophoresis Forum Agarose Gel Electrophoresis Forum. A forum to discuss electrophoresis staining, troubleshooting, and development of films for DNA and RNA gels.


co - migration of PCR products

co - migration of PCR products - Agarose Gel Electrophoresis Forum

co - migration of PCR products - Agarose Gel Electrophoresis Forum. A forum to discuss electrophoresis staining, troubleshooting, and development of films for DNA and RNA gels.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 09-07-2010, 11:50 AM
Pipette Filler
Points: 1,287, Level: 20 Points: 1,287, Level: 20 Points: 1,287, Level: 20
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Sep 2010
Posts: 3
Thanks: 0
Thanked 0 Times in 0 Posts
Default co - migration of PCR products



Hi,
i have a question.
I have two PCR amplicons of nearly identical size (173 and 186 bp) received by multiplex PCR and i'm interested if it is possible that they co - migrate in agarose gel electrophoresis. When there is only 186 bp product in the gel its position is a little bit higher than when there are this two PCR amplicons (173 and 186 bp) together.

Do you know something about this phenomena? thank you for yours replies
Reply With Quote
  #2  
Old 09-07-2010, 08:32 PM
luisillo's Avatar
M.D/Ph.D
Points: 3,877, Level: 41 Points: 3,877, Level: 41 Points: 3,877, Level: 41
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jul 2010
Location: Mexico
Posts: 352
Thanks: 19
Thanked 98 Times in 90 Posts
Default Re: co - migration of PCR products

If you want your fragments to separate enough to be visualizable you will have to increased the agarose concentration of you gel, that way the bands will appear less diffuse (or more compact, whatever) being easier to identify both of them. Also, run the electrophoresis for more time so that the fragments be able to separate form each other (both conditions, gel conc and time, have to be standarized).

By the way, polyacrilamide gels gives you better resolution for fragment separation.
Reply With Quote
Reply

Tags
migration , pcr , products


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Reaction mechanism and reaction products zhangtao@gmail.com Organic Chemistry Forum 0 12-19-2005 09:07 AM
A question for all the natural products afficionados out there Dirk Bussiere Botany Forum 13 10-17-2004 02:03 AM
Drosophila, cell migration, postdoctoral position in Seville Jose Casal Drosophila Forum 0 11-05-2003 06:59 PM
The Market for SNP Genotyping Products & Services: Enabling Pharmacogenomics Laura Wood Protocols and Methods Forum 0 09-17-2003 09:58 AM
The Market for SNP Genotyping Products & Services: Enabling Pharmacogenomics Laura Wood Microbiology Forum 0 09-17-2003 09:55 AM


All times are GMT. The time now is 09:20 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11916 seconds with 16 queries