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Does guanidinium thiocyanate denature RNA molecules or just the RNase enzymes?

Does guanidinium thiocyanate denature RNA molecules or just the RNase enzymes? - Agarose Gel Electrophoresis Forum

Does guanidinium thiocyanate denature RNA molecules or just the RNase enzymes? - Agarose Gel Electrophoresis Forum. A forum to discuss electrophoresis staining, troubleshooting, and development of films for DNA and RNA gels.


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Old 08-25-2010, 11:06 AM
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Default Does guanidinium thiocyanate denature RNA molecules or just the RNase enzymes?



Does guanidinium thiocyanate denature RNA molecules or just the RNase enzymes?
I'm pretty sure its just the enzymes....
Thanks,
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Old 08-25-2010, 07:43 PM
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Default Re: Does guanidinium thiocyanate denature RNA molecules or just the RNase enzymes?

Indeed, you are right. It denatures proteins. I donīt think it messes with hydrogen bonds, so it shouldnīt denature RNA, supossing your refering to RNA secondary structure.
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Old 02-22-2013, 05:12 PM
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Default Re: Does guanidinium thiocyanate denature RNA molecules or just the RNase enzymes?

The RNA is kept intact only proteins, such RNases are inactivated.
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denature , enzymes , guanidinium , molecules , rna , rnase , thiocyanate


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