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Agarose Gel Electrophoresis Forum Agarose Gel Electrophoresis Forum. A forum to discuss electrophoresis staining, troubleshooting, and development of films for DNA and RNA gels.


Molecular Bio help needed - Electrophoresis

Molecular Bio help needed - Electrophoresis - Agarose Gel Electrophoresis Forum

Molecular Bio help needed - Electrophoresis - Agarose Gel Electrophoresis Forum. A forum to discuss electrophoresis staining, troubleshooting, and development of films for DNA and RNA gels.


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Old 08-18-2010, 11:27 AM
Pipette Filler
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Default Molecular Bio help needed - Electrophoresis



Hi,
I'm trying to figure out a question for homework, it basically asks how it is possible to see plasmid bands when the stain (gel red) was not put in the lane of the plasmid in question?
* In another lane in the same gel the sample did get gel red added to it
any ideas?

thanks

* sorry reposted question in this section after i noticed this was a better area
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Old 08-25-2010, 08:02 AM
Pipette Filler
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Default Re: Molecular Bio help needed - Electrophoresis

Quote:
Originally Posted by mysticalelement View Post
Hi,
I'm trying to figure out a question for homework, it basically asks how it is possible to see plasmid bands when the stain (gel red) was not put in the lane of the plasmid in question?
* In another lane in the same gel the sample did get gel red added to it
any ideas?

thanks

* sorry reposted question in this section after i noticed this was a better area
I'm not quite sure what you mean? Normally DNA stains aren't added to individual lains, they're either mixed into the gel before casting, added to the running buffer or the gel is stained after the electrophoresis is run.
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