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|Agarose Gel Electrophoresis Forum Agarose Gel Electrophoresis Forum. A forum to discuss electrophoresis staining, troubleshooting, and development of films for DNA and RNA gels.|
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EtBr 'stuck' in gel
Hey, I've been running gels nearly non-stop for the past week or so, and had an unusual problem yesterday, that no one in the lab can fathom!
When I put the gel in the transluminator, I was getting a really bright 'splodge' which at first I thought was due to someone fiddling with the camera settings, or then maybe a lens flare effect or something, but I couldn't get rid of it. I tried placing it on a UV slab for direct visualization, and realized that the splodge was a load of ethidium bromide that had just stuck in that location on the gel for some reason!
I prepared the gel as normal, mixing the EtBr into the molten agarose and swirling thoroughly before casting, so I have not idea why this happened! Anyone have any ideas? My scanner's bust, so I've taken a photo of the photo of the gel and uploaded that.
Re: EtBr 'stuck' in gel
Is the top edge of your gel directly above your cathode? Since EthBr runs towards the cathode, if the gel is above your cathode, then maybe they will stop migrating and accumulate there? Just a thought.
|The Following User Says Thank You to jlf For This Useful Post:|
|agarose , dna , etbr , ethidium bromide , gel , tuck|
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