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Gel marker showed blur/no band

Gel marker showed blur/no band - Agarose Gel Electrophoresis Forum

Gel marker showed blur/no band - Agarose Gel Electrophoresis Forum. A forum to discuss electrophoresis staining, troubleshooting, and development of films for DNA and RNA gels.


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Old 06-04-2010, 02:50 PM
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Default Gel marker showed blur/no band



I've ran a 1.1% agarose gel using RNA. After running the gel, i stained it in EtBr for 10 minutes. When i put it into the illuminator, i see no band, not even the marker! What could be the reason of this? I put the well at the negative side of tank.

The second time i did it, there were bands, but still, the marker is very blur. Cant see the separation. Is this due to my handling problem or gel preparation/concentration problem? Or its the marker's problem? How can i improve the separation?
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Old 06-04-2010, 04:01 PM
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Default Re: Gel marker showed blur/no band

Looks like an EtBr problem, in my opinion. Is the EtBr solution fresh? If not, it may likely be already "consumed".

Why don't you try adding the EtBr directly when melting the agarose? Works perfect
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