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Single smear for DNA markers and ladders - HELP

Single smear for DNA markers and ladders - HELP - Agarose Gel Electrophoresis Forum

Single smear for DNA markers and ladders - HELP - Agarose Gel Electrophoresis Forum. A forum to discuss electrophoresis staining, troubleshooting, and development of films for DNA and RNA gels.


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Old 05-26-2010, 12:10 AM
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Default Single smear for DNA markers and ladders - HELP



Hi all,

Am running several DNA markers and ladders on a 0.5% agarose gel at 70V. Using TAE buffer with SYBR green 1 pre-loaded in gel. I am getting one long smear for all samples. I have done this before without problems so am concerned why this is happening. Markers are only 4 weeks old and have been stored in refridgerator. Only difference from last time was I centifuged the samples breifly to get them at the bottom (they had been shaken during transit) plus I used some very old agarose while waiting for new stock.

Any help or explanations appreciated.

Thanx
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Old 06-04-2010, 04:11 PM
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Default Re: Single smear for DNA markers and ladders - HELP

Most markers work cool in 1% agarose, why don't you try running all the DNA in 1% Agarose gel for 25 minutes at 95V, just to check if the DNA is the problem?
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