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|Agarose Gel Electrophoresis Forum Agarose Gel Electrophoresis Forum. A forum to discuss electrophoresis staining, troubleshooting, and development of films for DNA and RNA gels.|
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DNA < 70bp
Can anyone tell me methods to separate small DNA fragments (68, 38,28, 11 bps). I tried using a 4% agarose gel in TAE buffer (as the label on 10 bp marker says so). However, I was not able to resolve the marker bands also properly. I tried to do it with and without stain in the gel. No improvement. Any suggestions for alternative methods are welcome. I am planning to do it instead in TBE buffer. Does it practically make any difference?
People who worked with such smaller DNA sizes, please share your practical experience.
Thanks a lot,
|< , 4% agarose , 70bp , dna , small dna|