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Agarose Gel Electrophoresis Forum Agarose Gel Electrophoresis Forum. A forum to discuss electrophoresis staining, troubleshooting, and development of films for DNA and RNA gels.


DNA < 70bp

DNA < 70bp - Agarose Gel Electrophoresis Forum

DNA < 70bp - Agarose Gel Electrophoresis Forum. A forum to discuss electrophoresis staining, troubleshooting, and development of films for DNA and RNA gels.


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Old 03-10-2010, 08:43 PM
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Default DNA < 70bp



Hi,

Can anyone tell me methods to separate small DNA fragments (68, 38,28, 11 bps). I tried using a 4% agarose gel in TAE buffer (as the label on 10 bp marker says so). However, I was not able to resolve the marker bands also properly. I tried to do it with and without stain in the gel. No improvement. Any suggestions for alternative methods are welcome. I am planning to do it instead in TBE buffer. Does it practically make any difference?

People who worked with such smaller DNA sizes, please share your practical experience.

Thanks a lot,
Sirisha.
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Old 05-10-2010, 11:57 AM
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Default Re: DNA < 70bp

You can always try to run your samples in a poly accrylamide gel.
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