DNA < 70bp

[sirisha]

Hi,

Can anyone tell me methods to separate small DNA fragments (68, 38,28, 11 bps). I tried using a 4% agarose gel in TAE buffer (as the label on 10 bp marker says so). However, I was not able to resolve the marker bands also properly. I tried to do it with and without stain in the gel. No improvement. Any suggestions for alternative methods are welcome. I am planning to do it instead in TBE buffer. Does it practically make any difference?

People who worked with such smaller DNA sizes, please share your practical experience.

Thanks a lot,
Sirisha.

[Drekketh]

You can always try to run your samples in a poly accrylamide gel.