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Bands fade in ISSR fingerprinting

Bands fade in ISSR fingerprinting - Agarose Gel Electrophoresis Forum

Bands fade in ISSR fingerprinting - Agarose Gel Electrophoresis Forum. A forum to discuss electrophoresis staining, troubleshooting, and development of films for DNA and RNA gels.


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Old 02-26-2010, 10:55 AM
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Smile Bands fade in ISSR fingerprinting



I am working on ISSR fingerprinting. And am facing the problem of bands fading after running them for say 1/2 gel length. What may be the problem and solution for this.
thanks
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Old 02-26-2010, 04:01 PM
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Default Re: Bands fade in ISSR fingerprinting

It is probably that your staining solution is old.
Or you have bad buffer (too concentrated or wrong pH) and staining solution (with or without problems of its own).

Solution: remake both staining solution and electrophoresis/gel casting buffer.
Make sure they are made correctly.
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Old 03-01-2010, 06:13 AM
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Default Re: Bands fade in ISSR fingerprinting

Buffer is OK. I have checked the pH of TBA (5X). It is 8.4.
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