Loading limit on Agarose Gel

[charismafull]

I need to digest 15ug DNA with an restriction endonuclease enzyme of 4U/ ug DNA. My Stock DNA is 2.5 ug/ul and my stock enzyme is 5U/ul.* My buffer is 10x. My gel can only allow 64 ul of the total volume of the entire reaction.* I end up having 120ul which is twice the allowable volume.* If I force this process, I will end up having a smear instead of having a good band.* I can't even divide my DNA to digest into 2.* What should I do?

[kashir]

I think you can concentrate the DNA in a vacuum concentrator so that the volume becomes less and you have more DNA per microlitre.

[Drekketh]

It is realy simple to load excessive amounts of a sample in an agarose gel. I always load something of 200-400 ul of my digested samples when I want to linearise the vector, or to liberate a chosen fragment. The trick is:
Before you cast the gel (pour melted agarose in the gel tank), cover a few wells of your gel comb with a sealing tape, there you go you have bigger well