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Agarose Gel Electrophoresis Forum Agarose Gel Electrophoresis Forum. A forum to discuss electrophoresis staining, troubleshooting, and development of films for DNA and RNA gels.


DNA load limit in agrose gel

DNA load limit in agrose gel - Agarose Gel Electrophoresis Forum

DNA load limit in agrose gel - Agarose Gel Electrophoresis Forum. A forum to discuss electrophoresis staining, troubleshooting, and development of films for DNA and RNA gels.


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Old 02-05-2010, 07:47 PM
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Smile DNA load limit in agrose gel



I am trying to extract DNA from agrose gel. Is there a upper load limit of DNA per well? EX., the volume limit of one well is 100 microliters, there is 15microgram of DNA inside, is this overloaded or not? BTW, the buffer is 1XTAE, the gel is 0.5% agrose gel.
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