Hey, I need help in knowing how to determine the amount of concentration DNA that is present after doing gel electrophoresis.
I performed a gel with 3 different volumes of the same marker and 3 different volumes of the same sample of DNA.
The 3 volumes are 12ul, 6ul and 3 ul for both parts.
By visual comparison my 12ul of sample DNA is visually similar to the 6ul of the marker. I was wondering how to determine the concentration of DNA given that and some ot her information:
Sample DNA was orginally a plasmid that had been cut and the density is 1ug/ul.
The amount of sample DNA I had was 66uL.
Can someone please help me to determine the concentration of DNA there is in the 12ul band?