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Why no Visible Bands for Plant RNA Analysis??

Why no Visible Bands for Plant RNA Analysis?? - Agarose Gel Electrophoresis Forum

Why no Visible Bands for Plant RNA Analysis?? - Agarose Gel Electrophoresis Forum. A forum to discuss electrophoresis staining, troubleshooting, and development of films for DNA and RNA gels.


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Old 10-25-2009, 07:16 PM
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Default Why no Visible Bands for Plant RNA Analysis??



Hello biochemists,

Could any one explain how northern blotting can be used to analyse gene expression, Please ?

Actually, I did RNA extraction from plant last week. all extractions showed optical absorbance readings, yet some of the samples which
showed optical absorbance readings did not show any bands on the gel. Why might this be?

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1-photo taken 2 hrs after the gel was loaded
2- photo taken after the curent and voltage was reduced and the gel run overnight
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