Hi, I'm using a 4% gel to run 8 samples that vary by buffer and 2 ladders (25 and 100bp) at 130 volts for 30 min.
My question is: what would cause a lack of bands to show up? I've tried twice and I still have nothing. Someone else working in my lab got bands to show, so it cant be EtBd or any other products used in the process. Does anyone know of any common errors that might make a gel not run properly? I'm wondering if I'm making my gel wrong, but it doesn't appear odd.
I'm not expecting anyone to read this and be able to post something like "This is definitely what you're doing wrong..." but I'm an undergrad student and I'd really like to walk in on Tuesday with a good idea of what I may need to change to make my gel work.