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how much sample should i load if i'm using genomic DNA?

how much sample should i load if i'm using genomic DNA? - Agarose Gel Electrophoresis Forum

how much sample should i load if i'm using genomic DNA? - Agarose Gel Electrophoresis Forum. A forum to discuss electrophoresis staining, troubleshooting, and development of films for DNA and RNA gels.


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Old 04-17-2009, 03:01 AM
Pipette Filler
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Default how much sample should i load if i'm using genomic DNA?



hi, i'm a 3rd year biotech student. i'm currently extracting the DNA from the Phyllanthus plant. After i obtainedn the pellet of my DNA, i suspended it in 50ul of TE buffer. Then i added another 250ul of TE buffer in my stock. So what is actually my dilution factor? When i measure using Spectrophotometry, i took 1ul of sample and diluted it in 49ul of deionized water. the concentration of nucleic acid i obtained was 0.9570. how should i determine how much sample to load in the agarose gel? should i dilute the sample and how?
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