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-   -   Gel Streaking (http://www.molecularstation.com/forum/agarose-gel-electrophoresis-forum/65516-gel-streaking.html)

Lisa 04-06-2009 02:48 AM

Gel Streaking
 
I have been getting some streaking in my gels. I initially thought it was degradation, but have had the same result using fresh material. My PCR reactions are 20 microliters and I load about 2 or 3 microliters. Does anyone know what could be causing this?

Thanks a bunch!
Lisa

admin 04-06-2009 05:55 AM

Re: Gel Streaking
 
Hi Lisa,
welcome to the site. Can you post an image of the gel?

Sounds like a buffer or gel issue. Have you tried another buffer TAE or TBE / fresher buffer? What about gel voltage, you may be heating up your gel, is it hot when it comes out?

Kabqousain 04-02-2011 01:25 PM

Re: Gel Streaking
 
I am also facing the same problem of vertical streaks which are clearly visible between the DNA and RNA bands on the gel, the gel was not hot but i ran the samples for 20 minute at 150V,I used TBE buffer for this, I am a beginner so needs appropriate guidance that what can be the probable reason for it, may I use the same DNA samples for PCR or they have some degradation or other sort of abnormality in them?

Thanks

Amey5 08-03-2011 02:42 PM

Re: Gel Streaking
 
People,I have also been getting streaking in my gel......i am using qPCR and loading the PCR products in a gel gives me streaking near the wells .......is there a problem in the reaction or the gel???

thanks in advance


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