Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Molecular Biology Techniques > Agarose Gel Electrophoresis Forum
Register Search Today's Posts Mark Forums Read

Agarose Gel Electrophoresis Forum Agarose Gel Electrophoresis Forum. A forum to discuss electrophoresis staining, troubleshooting, and development of films for DNA and RNA gels.


Microsatellite (SSR)- Agarose Gel or Polyacrylamide Gel

Microsatellite (SSR)- Agarose Gel or Polyacrylamide Gel - Agarose Gel Electrophoresis Forum

Microsatellite (SSR)- Agarose Gel or Polyacrylamide Gel - Agarose Gel Electrophoresis Forum. A forum to discuss electrophoresis staining, troubleshooting, and development of films for DNA and RNA gels.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 01-05-2009, 01:23 PM
Volunteer
Points: 3,015, Level: 35 Points: 3,015, Level: 35 Points: 3,015, Level: 35
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2006
Posts: 43
Thanks: 0
Thanked 3 Times in 3 Posts
Default Microsatellite (SSR)- Agarose Gel or Polyacrylamide Gel



Hello all!
I'm planning to do SSRs marker. Should I use 2% agarose gel to detect DNA fragments or should I use polyacrylamide (PAA).

Thanks in advance
Reply With Quote
  #2  
Old 01-05-2009, 01:27 PM
admin's Avatar
Administrator
 
Join Date: Nov 2005
Posts: 1,418
Thanks: 883
Thanked 68 Times in 58 Posts
Default Re: Microsatellite (SSR)- Agarose Gel or Polyacrylamide Gel

Hi there DNAmolecule!
Happy New Year!
If your difference in microsatellites is more (10-11 bp) you can use 3% agarose, but if it is less then do PAGE. Metaphor gel is also good to use. In pAGE the difference and ease to score is worth doing.

Hope this helped!
Cheers
Reply With Quote
  #3  
Old 01-27-2009, 12:26 PM
Pipette Filler
Points: 6, Level: 1 Points: 6, Level: 1 Points: 6, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2009
Posts: 4
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Microsatellite (SSR)- Agarose Gel or Polyacrylamide Gel

Quote:
Originally Posted by dnamolecule View Post
Hello all!
I'm planning to do SSRs marker. Should I use 2% agarose gel to detect DNA fragments or should I use polyacrylamide (PAA).

Thanks in advance
We suggest you to use Elchrom Scientific gels which are not acrylamide but same resolution (up to 1 bp) and not toxic!!! For further information look at
Elchrom Scientific homepage (type the name in google)
where you can find also reference on Microsatellite.
Reply With Quote
  #4  
Old 01-27-2009, 12:28 PM
Pipette Filler
Points: 6, Level: 1 Points: 6, Level: 1 Points: 6, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2009
Posts: 4
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Microsatellite (SSR)- Agarose Gel or Polyacrylamide Gel

Use Elchrom Scientific gels. Same resolution of acrylamide (up to 1 bp) but precast and not toxic!! Go to Elchrom Scientific homepage (via google) and look at the many references on Microsatellite.
Reply With Quote
Reply

Tags
agarose , gel , microsatellite , polyacrylamide , ssr


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Agarose gel electrophoresis of PCR products - Method and Protocol domba PCR - Polymerase Chain Reaction Forum 12 02-24-2012 03:39 AM
agarose gel purification of cloning vector mwcrepeau@hawaii.rr.com Protocols and Methods Forum 5 06-17-2010 09:33 AM
Sybr Green II and denaturing agarose gels heliozoan RNA Techniques Forum 2 05-11-2008 05:23 AM
EtBr in polyacrylamide gels Jayakumar, R Protocols and Methods Forum 0 04-29-2006 04:37 AM
Nusieve agarose gel Samantha Protocols and Methods Forum 1 11-05-2004 10:32 AM


All times are GMT. The time now is 11:20 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.13116 seconds with 16 queries