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isolation of plasmid

isolation of plasmid - Agarose Gel Electrophoresis Forum

isolation of plasmid - Agarose Gel Electrophoresis Forum. A forum to discuss electrophoresis staining, troubleshooting, and development of films for DNA and RNA gels.


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Old 08-10-2008, 09:33 AM
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Default isolation of plasmid



Hi all,
I ran a gel of isolated plasmids into 8 wells.4 of the wells gave bright glow colour which indicates the abundance of plasmids (am i right?).4 others gave faint uniform bands (looking as though they were digested even though they are not).pls i dont understand.if u want me to send the pictures i would.Help
Thanks
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Old 08-11-2008, 10:14 AM
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Default Re: isolation of plasmid

Hi, send me the gel picture, I will give some suggestions
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Old 08-11-2008, 10:45 AM
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Default Re: isolation of plasmid

Hello Row,
you can post your gel picture here by attaching the picture (go Advanced - button below) then Attach browse to your picture and click submit.

You may have bad DNA preps for those 4 or DNAse contamination / degradation of your DNA.
Did you isolate them differently or store them differently?
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Old 08-19-2008, 02:57 PM
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Default Re: isolation of plasmid

i am sorry for my late reply.i actually did not know how to operate the reply thread until now.i am sending the attachment.pls when the lanes have a convex end instead of a straight one.is it significant.Remember i have neither digested or ligated them.i just put them straight in the Ecoli.Can i detect shapes of the plasmids from these lanes.Thanks
N.B i am having a problem compressing the file.Do you have an email i can send it to?
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