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help - Agarose Gel Electrophoresis Forum

help - Agarose Gel Electrophoresis Forum. A forum to discuss electrophoresis staining, troubleshooting, and development of films for DNA and RNA gels.


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  #1  
Old 11-08-2008, 02:30 AM
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after my run and withen 10 min. all results fade from gel ,i use new ethedium ,any one has explanation
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  #2  
Old 12-01-2008, 02:26 AM
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Default Re: help

me also, I have the same problem.But i think it is the agarose type!!
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  #3  
Old 12-08-2008, 05:04 PM
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Default Re: help

have u change the agarose and observe any difference? was the 10 mins is continual expose to UV light?
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Old 12-09-2008, 09:59 PM
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Default Re: help

yes, it is exposed to uv and i havnt change the agarose
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  #5  
Old 12-10-2008, 08:09 AM
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Default Re: help

exposure to uv for 10mins?! no wonder it fade. well usually taking gel photo i took few sec and decrease time of uv exposure. as uv degrade it. just on the uv when about to snap photo and off uv when done.
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Old 12-17-2008, 06:37 PM
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Default Re: help

I changed agarose and no fading occured thankes alot butters
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  #7  
Old 12-20-2008, 08:09 AM
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Default Re: help

wow dr.dalia was right! if possible can you tell us what agarose brand is that? so for those who dont want to repeat ur trouble can avoid. and maybe some like to re-test it
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Old 12-23-2008, 07:09 AM
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Default Re: help

u expore gel overlong.
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