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PCR - Polymerase Chain Reaction Forum PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.

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PCR - Polymerase Chain Reaction Forum


Rt-pcr

PCR - Polymerase Chain Reaction Forum

PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.



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  #1 (permalink)  
Old 07-15-2008, 05:21 AM
Pipette Filler
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Default Rt-pcr
In RT-PCR ,wih bandf of my intrest we also got two extra light band.how can i improve my result.should i decrease my primer concentration?or should i change annealing temperature?
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  #2 (permalink)  
Old 07-16-2008, 09:20 AM
Pipette Filler
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Default Re: Rt-pcr
increase the temperature and decrease the primer conc
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