Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Proteomics Forum > 2-D Gel Electrophoresis Forum
Register Search Today's Posts Mark Forums Read

2-D Gel Electrophoresis Forum 2-D Gel Electrophoresis Forum. A forum dedicated to two dimensional gel electrophoresis, discussion about systems, and troubleshooting its problems.


WHY,the less protein spot?

WHY,the less protein spot? - 2-D Gel Electrophoresis Forum

WHY,the less protein spot? - 2-D Gel Electrophoresis Forum. A forum dedicated to two dimensional gel electrophoresis, discussion about systems, and troubleshooting its problems.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 04-21-2012, 03:12 AM
Pipette Filler
Points: 188, Level: 3 Points: 188, Level: 3 Points: 188, Level: 3
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Mar 2012
Location: Beijing,China
Posts: 2
Thanks: 0
Thanked 0 Times in 0 Posts
Post WHY,the less protein spot?



I have a problem,when i have extracted plant protein,my partner help me extract one sample,the others was extracted by myself.The picture of the 2D gel was that my partner's good,but mine have less protein spots.All of methods are the same,I use TCA-acetone.I ground the plant sample use over five minutes,I guess the protein is degraed.Please help.

Last edited by johnyshyoung; 04-21-2012 at 03:14 AM.
Reply With Quote
Reply

Tags
protein , spot


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Human Cytome Project - Update 24 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 1 08-01-2010 02:18 PM
Human Cytome Project - an idea - Update 19 April 2005 Peter Van Osta Cell Biology and Cell Culture 1 06-01-2009 02:17 PM
Human Cytome Project - Update 6 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 0 01-06-2005 11:18 AM
New Saccharomyces Sequences 11/27/04 Mike Cherry Yeast Forum 0 11-29-2004 12:39 AM
New Saccharomyces Sequences 05/19/04 SGD Sequences Yeast Forum 0 05-23-2004 04:06 PM


All times are GMT. The time now is 02:35 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.15862 seconds with 16 queries