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What are the protein modifications shifts the pI to basic side?

What are the protein modifications shifts the pI to basic side? - 2-D Gel Electrophoresis Forum

What are the protein modifications shifts the pI to basic side? - 2-D Gel Electrophoresis Forum. A forum dedicated to two dimensional gel electrophoresis, discussion about systems, and troubleshooting its problems.


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Old 09-01-2011, 02:13 PM
Pipette Filler
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Default What are the protein modifications shifts the pI to basic side?



Hi,
Anybody knows , what are the modifications shifts the protein to basic acid?

I know phosphorylation and acetylation shifts it to acidic side, any other modification for acidic side?

and What are the modifications shifts the molecular weight change 5 -10kDa difference?

I have protein spots 5 to 6 spots in basic side in 2D westernblot with 2 to 5 kDa differences . what it could be?

thx
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