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|2-D Gel Electrophoresis Forum 2-D Gel Electrophoresis Forum. A forum dedicated to two dimensional gel electrophoresis, discussion about systems, and troubleshooting its problems.|
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Hi guys, I'm testing two different IEF protocols, and I need some advices.
My strips are 18 cm longer, pH 3-10. I have changed GE's standart protocol to improve my focalization, but I don't think it is good enough.
Is it better a long time in low voltages (i.e. 1000 V step) with many volt hours, or a longer time in higher voltages (i.e. 10000V step)?
The attachments contain the two protocols tested.
|2d electrophoresis , doubt , ief , opinion , proteomics|
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