Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Proteomics Forum > 2-D Gel Electrophoresis Forum
Register Search Today's Posts Mark Forums Read

2-D Gel Electrophoresis Forum 2-D Gel Electrophoresis Forum. A forum dedicated to two dimensional gel electrophoresis, discussion about systems, and troubleshooting its problems.


IEF opinion

IEF opinion - 2-D Gel Electrophoresis Forum

IEF opinion - 2-D Gel Electrophoresis Forum. A forum dedicated to two dimensional gel electrophoresis, discussion about systems, and troubleshooting its problems.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 05-24-2011, 03:45 PM
Pipette Filler
Points: 46, Level: 1 Points: 46, Level: 1 Points: 46, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2011
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Question IEF opinion



Hi guys, I'm testing two different IEF protocols, and I need some advices.
My strips are 18 cm longer, pH 3-10. I have changed GE's standart protocol to improve my focalization, but I don't think it is good enough.
Is it better a long time in low voltages (i.e. 1000 V step) with many volt hours, or a longer time in higher voltages (i.e. 10000V step)?
The attachments contain the two protocols tested.
Thanks!
Reply With Quote
Reply

Tags
2d electrophoresis , doubt , ief , opinion , proteomics


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
In your opinion, what are the top 5 rarest (nearly extinct) or most... Chuka Zoology Forum 0 06-23-2009 08:17 PM
Global Warming? Your opinion against mine.? Courtlyn Environmental Sciences and Issues 0 06-23-2009 07:16 AM
please answer best answer ten points. will answer in return. own opinion? Shorty S Biology Forum 2 06-22-2009 10:17 PM
what is your opinion on global warming? are we really destroying the planet? Ashley Environmental Sciences and Issues 1 06-22-2009 11:17 AM
Please send me your opinion about my project as placed on inforelation4 Botany Forum 0 11-30-2004 02:27 PM


All times are GMT. The time now is 11:39 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11527 seconds with 17 queries