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Problems with high molecular weight proteins

Problems with high molecular weight proteins - 2-D Gel Electrophoresis Forum

Problems with high molecular weight proteins - 2-D Gel Electrophoresis Forum. A forum dedicated to two dimensional gel electrophoresis, discussion about systems, and troubleshooting its problems.


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Old 12-10-2010, 05:21 PM
Pipette Filler
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Question Problems with high molecular weight proteins



Please we are having the same problem in the lab with some different samples.
Proteins of high molecular weight are not seen at all in the gel... I m sending attached an example... have you of you already experience something like that?
Thanks a lot!
;-)
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Old 12-13-2010, 07:09 PM
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Default Re: Problems with high molecular weight proteins

Did the gel polymerized well? The running looks very irregular
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Old 05-30-2011, 07:17 AM
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Default Re: Problems with high molecular weight proteins

Luisillo has pointed currectly! Your IEF is very good, but the polymerization is irregular. Please add 40 microLiter 20% APS and 8 microLiter TEMED for a 12 ml gel mixture and pour immediately. lay a hundred microLiter of distilled water on top. leave for at least 45 minutes to polymerize.
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