How to optimize IEF program parameters?

janemon · #1 08-21-2010, 02:45 PM

Like, when to use step and hold and when to use gradient? Thank you.

dinesh2dg · #2 03-18-2011, 12:10 PM

Hi Janemon,
i also have the same question....if you know, plz let me know.......

arnab_roy · #3 05-30-2011, 07:04 AM

I can only say that I have used exactly what is instructed in the product manual, from Biorad. It mentions all parameters for each kind of IPG strip. First step is to follow these strictly. If there is scope for improvement, one can change the specific parameter that might be helpful.

I have got pretty good results from the prescribed parameters.

Cheers!

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How to optimize IEF program parameters?

How to optimize IEF program parameters? - 2-D Gel Electrophoresis Forum

How to optimize IEF program parameters? - 2-D Gel Electrophoresis Forum. A forum dedicated to two dimensional gel electrophoresis, discussion about systems, and troubleshooting its problems.