Hello, i am new here. I desperately need your helps people. basically i ve done all the steps and desalted all my proteins before loading, i am running 100 μg of protein and bad enough during my active rehydration step for 12 hours. the voltage reading is noraml at 50V
However, the other read 0μA (was 50μA. 2 hours later is at 1 or 0) and 0W. My 3 months of work will be down the drain are there any solutions to avoid things from happening. i ve did many washes, presumably desalting the sample.
Anyways i am working on fungal secreted proteins. Could it be that everything will be alright? thx guys in need of desperate help now