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Choosing between stains

Choosing between stains - 2-D Gel Electrophoresis Forum

Choosing between stains - 2-D Gel Electrophoresis Forum. A forum dedicated to two dimensional gel electrophoresis, discussion about systems, and troubleshooting its problems.


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Old 01-04-2010, 06:42 PM
Kas Kas is offline
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Default Choosing between stains



Hi,

I have used silver staining and Brilliant Blue G Colloidal (Sigma). Even though the detection of the colloidal Blue G is less then the silver stain, the quality of the results was better. Now there is the possibility of using Bio-Safe Coomassie Stain (Bio-Rad). Apparentyly the sensitivity can be 8ng (the refered sensitivity of the rilliant Blue G Colloidal (Sigma) is 50ng. Has anyone compared the two? Is the bio-rad stain really better?

thanks for your help
Kalina
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