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Staining 2D Gels

Staining 2D Gels - 2-D Gel Electrophoresis Forum

Staining 2D Gels - 2-D Gel Electrophoresis Forum. A forum dedicated to two dimensional gel electrophoresis, discussion about systems, and troubleshooting its problems.


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Old 07-01-2009, 03:24 AM
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Question Staining 2D Gels



hello all.

i used silver staining for 2D gels but somehow my gels turn out to be very dark (making the spots hard to see). can anyone gives opinion/suggestion?

isn't necessary to wash the gel with ethanol after fixation (before sensitizing)?
and im thinking of reducing the time for sensitizing and developing steps.
ty
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