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IEF problem

IEF problem - 2-D Gel Electrophoresis Forum

IEF problem - 2-D Gel Electrophoresis Forum. A forum dedicated to two dimensional gel electrophoresis, discussion about systems, and troubleshooting its problems.


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Old 06-26-2009, 03:10 AM
Pipette Filler
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Default IEF problem



Hi, I am doing plant proteomics. I have a problem on my IEF as below:

1. My bromophenol blue is very difficult to reach to the end. What should I do?
I did TCA/Acetone protein precipitation on my sample.

2. After finished focusing, I notice that the gel was thicker at the both end of the strip. I am using strip pH 4-7, 7cm.

Thx

Hew
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