Hi, I am doing plant proteomics. I have a problem on my IEF as below:
1. My bromophenol blue is very difficult to reach to the end. What should I do?
I did TCA/Acetone protein precipitation on my sample.
2. After finished focusing, I notice that the gel was thicker at the both end of the strip. I am using strip pH 4-7, 7cm.
|All times are GMT. The time now is 02:35 AM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2015, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved