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-   -   IEF problem (http://www.molecularstation.com/forum/2-d-gel-electrophoresis-forum/68212-ief-problem.html)

hewchawsen 06-26-2009 03:10 AM

IEF problem
 
Hi, I am doing plant proteomics. I have a problem on my IEF as below:

1. My bromophenol blue is very difficult to reach to the end. What should I do?
I did TCA/Acetone protein precipitation on my sample.

2. After finished focusing, I notice that the gel was thicker at the both end of the strip. I am using strip pH 4-7, 7cm.

Thx

Hew


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