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Resuspend pellet in acid buffer

Resuspend pellet in acid buffer - 2-D Gel Electrophoresis Forum

Resuspend pellet in acid buffer - 2-D Gel Electrophoresis Forum. A forum dedicated to two dimensional gel electrophoresis, discussion about systems, and troubleshooting its problems.


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Old 04-18-2009, 10:08 AM
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Default Resuspend pellet in acid buffer



Hi, after tca precipitaion I need that my sample is in acid conditions to prevent free thiol re-shuffling.
What happen if I resuspend the protein pellet in Urea/thiourea in 10mM H3PO4 pH 2,2 or also in 10 mM acetate at pH 5?
Have I sample loss or preferencial protein recovery?
The sample is from mammalia cells.
Thank u
Giovanni

Last edited by giovanni; 04-18-2009 at 12:06 PM.
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