| | Re: IEF of plant proteins
Hi, I can tell you, that IPG 4-7 is radically better than IPG 3-10 and you can see probably the same range of proteins on both (the focusing on IPG 3-7 ends on pH 7 approximately). About ampholytes, I do not know, if I get your point, but in IPG lysis buffer I use the same ampholytes for both IPG strips. The important thing is: do you precipitate your proteins before loading on the strips? IPG 3-10 usually requires sample precipitation, specially if your sample contains big amounts of lipids or some others contamination. IPG 4-7 does not require this procedure.