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-   -   2nd dim: high-weight zone depleted (http://www.molecularstation.com/forum/2-d-gel-electrophoresis-forum/56231-2nd-dim-high-weight-zone-depleted.html)

lja 02-05-2009 11:15 PM

2nd dim: high-weight zone depleted
 
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Hi everybody,
I encounter a big main problem in 2-dim electrophoresis: all the high weight zone is depleted, like "erased" from the gel. It happens indipendently from the protein quantity and actually I suspect it is a problem of the 2nd dimension: of 12 tubes I ran for the 1st dimension, the first 4 SDS-PAGE were fine, the other 8, plus others I ran afterwards, present the same problem. I try to upload a phote, maybe it helps. Any hint will be welcome, I am currently stacking on this!
:worshippy:

admin 02-06-2009 07:43 AM

Re: 2nd dim: high-weight zone depleted
 
http://www.molecularstation.com/foru...c_050-jpeg-jpg


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