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Troubleshooting Streaking of 2-D Gels

Troubleshooting Streaking of 2-D Gels - 2-D Gel Electrophoresis Forum

Troubleshooting Streaking of 2-D Gels - 2-D Gel Electrophoresis Forum. A forum dedicated to two dimensional gel electrophoresis, discussion about systems, and troubleshooting its problems.


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Old 06-23-2008, 11:40 AM
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Question Troubleshooting Streaking of 2-D Gels



Hi all, I am having troubles with 2-D gels lately. My gels keep streaking vertically. Has anyone had this problem lately and have any solutions?

thanks
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Old 06-24-2008, 03:35 AM
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Smile Re: Troubleshooting Streaking of 2-D Gels

Quote:
Originally Posted by kiki06 View Post
Hi all, I am having troubles with 2-D gels lately. My gels keep streaking vertically. Has anyone had this problem lately and have any solutions?

thanks
Here's a nice troubleshooting guide ---> [Only registered users see links. ]

I solve my vertical streaking by reducing the total protein loaded.
But as the guide points out, longer equilibration time and fresh SDS electrophoresis buffer may solve the problem.
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Old 07-03-2008, 03:56 PM
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Default Re: Troubleshooting Streaking of 2-D Gels

thanks a lot for that guide! I have repeated it with the suggestions and it looks much better
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