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PCR help
hi there,
If I am going to do a PCR amplification of a region in the E. coli genome. I wonder if the first PCR melting temperature should be the...
DNA sequencing help
I am trying to perform sequencing on ABI 3130 sequencing using Bigdye v3.1. I want to do Ethanol/EDTA precipitation. The protocol says "...add EDTA...
ssDNA synthesis from PCR and Denaturing PAGE
I am trying to develop a method to synthesize 100-200 bases of ssDNA product from PCR. I have a template lambda DNA. presently using assymetric pcr i...
Suggestions on RNAse treatment after Genomic DNA Isolation
Hi guys i have extracted genomic DNA from various cell lines....kit i used had an optional step for RNAse treatment that i skipped. after taking OD...
DNA extraction Method from Blood
I am working in the field of Diabetes genetics. Please let me know which method is best to extract DNA from Blood sample, Here in may lab they have...
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