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DNA Station is a portal for molecular biology, specifically DNA, and includes DNA protocols, DNA bioinformatics, DNA epigenetics, and information about DNA - deoxyribonucleicacid, the hereditary molecule.
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Question regarding 70% EtOH in EtOH Precipitation
I mistakenly added 10% EtOH instead of 70% EtOH for an ethanol precipitation protocol. It wasn't until 6 hours later that I realized my mistake. Is...
PCR with 1 Primer
I'm trying to amplify an unsequenced region of DNA containing highly repetitive and highly polymorphic genes. I have obtained some good clean...
My experience with AccessDNA
Recetly I came across accessdna.com, a avery good site providing good information about genetic disorders, risks etc. If you sign up on AccessDNA,...
PCR help
hi there,
If I am going to do a PCR amplification of a region in the E. coli genome. I wonder if the first PCR melting temperature should be the...
DNA sequencing help
I am trying to perform sequencing on ABI 3130 sequencing using Bigdye v3.1. I want to do Ethanol/EDTA precipitation. The protocol says "...add EDTA...
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