Western Blot Western Blot
Membrane Stripping and Re-probing Protocol - Western Blotting Membrane Stripning og Re-prøver protokollen - Western blotting
Membrane Stripping and Reprobing Protocol for Western Blotting Membrane Stripning og Reprobing protokollen for Western blotting
Stipping and re-probing blots Stipping og re-prøver blotting
- add 280 μl of 2-beta-mercaptoethanol per 40 mL of stripping buffer (to a final concentration of 100 mM) tilføj 280 μ l af 2-beta-mercaptoethanol per 40 ml stripping buffer (til en slutkoncentration på 100 mm)
- Wash membrane in TBST (1 X 5 min) Vask membranen i TBST (1 x 5 min)
- Incubate membrane in stripping buffer for 30 min at 50-60°C (use a heating oven for this - and keep the oven door closed due to smell) Inkubér membranen i stripping buffer i 30 min ved 50-60 ° C (brug en varme ovn for dette - og holde ovnen døren lukket på grund af lugt)
- Wash in TBST (2 X 5 min) Vask i TBST (2 x 5 min)
- Block membrane for 1 h in 5% blocking milk Block membranen i 1 h på 5% blokering mælk
- Follow normal protocol for developing western blots. Følg normale protokol for at udvikle western blotting.
- Membrane Stripping Buffer Recipe: Membrane Stripning Buffer opskrift:
- Stripping buffer: 62.5 mM Tris-HCl (pH 6.8), 2% SDS. Stripning buffer: 62,5 mM Tris-HCl (pH 6.8), 2% SDS. (add 280 ul of 2-beta merceptoethanol or a final concentration of 0.1 M 2-Mecaptoethanol). (tilføj 280 ul af 2-beta merceptoethanol eller en endelig koncentration på 0,1 M 2-Mecaptoethanol).
- See our Western Blotting Protocol Se vores Western blotting-protokollen
Other Protein Protocols in our Protocol Database Andre Protein protokoller i vores protokol Database
Our Protein Protocols Vores Protein protokoller
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