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Molecular Station is THE largest network of researchers, scientists and science lovers anywhere! Molekylær Station er det største netværk af forskere, videnskabsmænd og videnskab kærester overalt!
A science is any discipline in which the fool of this generation can go beyond the point reached by the genius of the last generation. En videnskab er en disciplin, hvor narre af denne generation kan gå ud over det punkt nået frem til geni af den sidste generation. ~Max Gluckman, Politics, Law and Ritual, 1965 ~ Max Gluckman, Politik, Lov og Ritual, 1965
Peptides are quite expensive and it is wise if one reads about peptide storage and handling. Peptider er ret dyrt, og det er klogt, hvis man læser om peptid opbevaring og håndtering.
You will probably receive your peptides are air or freeze dried, fluffy pellets in serum vials. Du vil sandsynligvis modtage din peptider er luft eller frysetoerrede, fluffy pellets i serum hætteglas.
To reconstitute the peptide one can use either distilled water or buffer solution. For at rekonstruere peptid kan man bruge enten destilleret vand eller buffer løsning.
If your peptide has a low solubility in water, then it may have to be dissolved in a solvent. Hvis din peptid har en lav opløselighed i vand, så kan det være nødvendigt at opløses i et opløsningsmiddel.
Other solvents used to dissolve peptides include: Andre opløsningsmidler, der anvendes til at opløse peptider omfatter:
Whatever you use, make sure you keep your peptide concentrated. Uanset hvad du bruger, skal du sørge for at holde din peptid koncentreret. It is quite difficult and a waste of expensive peptide to try and reconcentrate the Det er ganske vanskelig og spild af kostbar peptid at forsøge at reconcentrate de
Use the minimal amount of these solvents, distilled purified water or buffer to make up your peptide stock solution. Brug minimalt beløb for disse opløsningsmidler, destilleret renset vand eller buffer til at gøre din peptid stamopløsning.
Store peptides in usually stored in the - 20°C freezer immediately after they are received. Store peptider i normalt gemmes i - 20 ° C fryser umiddelbart efter de er modtaget.
After re-solubilization, peptides should be used immediately to avoid peptidase and protease degradation in solution. Efter re-solubilization, peptider bør anvendes straks for at undgå peptidase og protease nedbrydning i opløsning. Peptide should be aliquoted if it all won't be used immediately or stored. Peptide bør aliquoted hvis det hele ikke vil blive anvendt straks eller opbevares. A good idea is to aliquot it into single-use portions, and relyophilized if possible. En god ide er at prøve det i engangsinstrumenter portioner, og relyophilized hvis muligt. Then store it again in the freezer. Så gemme den igen i fryseren.
AVOID Repeated thawing and refreezing of peptide. Undgå at gentagne optøning og genindfrysning af peptid.
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