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Gene Cloning Gene Cloning

Information on how genes, (usually proteins) or cells are cloned. Oplysninger om, hvordan gener, (oftest proteiner) eller celler klonet.

Gene Cloning Method Gene Cloning Metode

The purpose of any cloning experiment is to produce a clone, a group of identical cells or organisms.  We know that some plants can be cloned simply by taking cuttings, and that others can be cloned by growing whole plants from single cells collected from one plant. Formålet med enhver kloning eksperiment er at producere en klon, en gruppe af identiske celler eller organismer. Vi ved, at nogle planter kan klonede blot ved at tage stiklinger, og at andre kan blive klonet af voksende hele planter fra enkelt celler indsamlet fra en plante .

Even vertebrates can be cloned.  John Gurdon produced clones of identical frogs by transplanting nuclei from a single frog embryo to many enucleate eggs, and a sheep named Dolly was cloned in Scotland in 1997 using an enucleate egg and a nucleus from an adult sheep mammary gland.  Identical twins constitute a natural clone. Selv hvirveldyr kan klones. John Gurdon fremstillet kloner af identiske frøer ved transplantation af cellekerner fra en enkelt Frøen foster til mange enucleate æg, og et får navnet Dolly blev klonet i Skotland i 1997 ved hjælp af en enucleate æg og en cellekerne fra et voksent får brystkirtel . Enæggede tvillinger udgør en naturlig klon.

The procedure we usually follow in a gene cloning experiment is to place a foreign gene into a bacterial cell, then to grow a clone of these modified bacteria, with each bacterial cell containing the foreign gene.  Thus, as long as we ensure that the foreign gene can replicate, we can clone the gene by cloning its bacterial host.  Stanley Cohen, Herbert Boyer, and their colleagues performed the first cloning experiment in 1973. Den procedure, vi normalt følger i et gen kloning eksperimentet er at placere et fremmed gen ind i en bakteriel celle, derefter at stige en klon af disse modificerede bakterier, med hver bakteriel celle indeholdende fremmed gen. Derfor, så længe vi sikrer, at den udenlandske gen kan kopiere, kan vi klone det gen ved kloning sin bakteriel vært. Stanley Cohen, Herbert Boyer, og deres kolleger udførte den første kloning eksperiment i 1973.

Related Gene Cloning Articles: Relaterede Gene Cloning artikler:

DNA Cloning DNA Kloning

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