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Immunology Home Immunology Home

At Molecular Station Immunology Home you will find information, protocols, bioinformatics, and links on: På Molekylær Station Immunology Hjem vil du finde oplysninger, protokoller, bioinformatik og links på:

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Latest Research in the Field of Immunology from the Journal Immunology, PubMed: Seneste forskning inden for Immunology fra Journal Immunology, PubMed:

Regional variations in Paneth cell antimicrobial peptide expression along the... Regionale forskelle i Paneth celle antimikrobielle peptid udtryk langs ...

Regional variations in Paneth cell antimicrobial peptide expression along the mouse intestinal tract. Regionale forskelle i Paneth celle antimikrobielle peptid udtryk langs musen tarmkanalen.

BMC Immunol. BMC Immunol. 2008 Jul 17;9(1):37 2008 juli 17, 9 (1): 37

Authors: Karlsson J, Putsep K, Chu H, Kays RJ, Bevins CL, Andersson M Forfattere: Karlsson J, Putsep K, Chu H, Kays RJ, Bevins CL, Andersson M

ABSTRACT: BACKGROUND: Enteric antimicrobial peptides secreted from Paneth cells, including alpha-defensins (in mice named cryptdins), are key effector molecules of innate immunity in the small intestine. Resumé: BAGGRUND: Enteric antimikrobielle peptider udskilles fra Paneth celler, herunder alfa-defensins (i mus navngivne cryptdins), er centrale effector molekyler af medfødte immunitet i tyndtarmen. The importance of Paneth cells alpha-defensins emerged from studies of enteric bacterial infection in genetically modified mice, as well as from recent studies linking reduced levels of these alpha-defensins to Cohn's disease localized to the ileum. Betydningen af Paneth celler alpha-defensins opstået fra undersøgelser af enterisk bakteriel infektion i genetisk modificerede mus, samt fra nyere undersøgelser forbinder nedsatte niveauer af disse alpha-defensins til Cohn's sygdom lokaliseret til ileum. However, analysis of expression of Paneth cell alpha-defensins is incomplete. Men analysen af udtryk for Paneth celle alpha-defensins er ufuldstændige. We therefore performed a comprehensive evaluation of the distribution of antimicrobial molecules along the mouse small intestinal tract to identify potential variations in regional expression. Vi har derfor foretaget en omfattende evaluering af distribution af antimikrobielle molekyler langs musen små tarmkanalen til at identificere potentielle variationer i regionale udtryk. RESULTS: In conventionally reared mice, the repertoire of Paneth cell antimicrobials differs between duodenum and ileum. Resultater: I konventionelt opdrættet mus, repertoire af Paneth celle antimikrobielle varierer mellem duodenum og ileum. In contrast to the uniform expression of most Paneth cell antimicrobials, both cryptdin 4 and cryptdin-related sequences (CRS) 4C peptides were expressed at progressively increasing amounts (10exp1- and 10exp4-fold, respectively) comparing duodenum and ileum. I modsætning til de ensartede udtryk for de fleste Paneth celle antimikrobielle stoffer, både cryptdin 4 og cryptdin-relaterede sekvenser (CRS) 4C peptider blev udtrykt på gradvis stigende beløb (10exp1-og 10exp4 gange, respektivt) sammenligne duodenum og ileum. In tissues other than the small intestine, expression of CRS peptides was noted in thymus and caecum. I væv, bortset fra tyndtarmen, udtryk for CRS peptider blev bemærket i brissel og blindtarm. Most Paneth cell products were also produced in the small intestine of germ-free mice at levels similar to those in controls, however CRS4C and RegIIIgamma had reduced levels in the former (3- and 8-fold, respectively). De fleste Paneth celle produkter blev også produceret i tyndtarmen af kim-frie mus på et niveau svarende til dem i kontrol, dog CRS4C og RegIIIgamma var lavere i den tidligere (3 - og 8-fold, respectively). No significant changes in expression levels of Paneth cell antimicrobial peptides was observed after oral challenge with either Salmonella enterica serovar typhimurium or Listeria monocytogenes, supporting current notions on the constitutive nature of this defensive system. Ingen signifikante ændringer i udtrykket niveauer af Paneth celle antimikrobielle peptider blev observeret efter oral udfordring med enten Salmonella enterica serovar typhimurium eller Listeria monocytogenes, støtte aktuelle begreber om konstitutive karakter af denne defensive system. CONCLUSIONS: The repertoire of antimicrobial peptides changes along the small intestinal tract, and a subset of these molecules are up-regulated upon colonization, but not in response to enteric bacterial pathogens. Konklusioner: Det repertoire af antimikrobielle peptider ændringer langs de små tarmkanalen, og en delmængde af disse molekyler er op-reguleret på kolonisering, men ikke som svar på entero bakterielle smitstoffer. The changes detected upon colonization suggest that Paneth cell antimicrobial peptides may play an important role in commensal microbial homeostasis, in addition to their proposed role in protection against infection. De ændringer opdages ved kolonisering tyder på, at Paneth celle antimikrobielle peptider kan spille en vigtig rolle i commensal mikrobielle homøostase, i tillæg til deres foreslåede rolle i beskyttelse mod infektion. In addition, the differential expression of CRS4C along the small intestine suggests mechanisms of regulation that are distinct from other Paneth cell derived antimicrobial peptides. Desuden er forskellen udtryk for CRS4C langs tyndtarmen foreslår mekanismer for regulering, der er adskilt fra andre Paneth celle afledt antimikrobielle peptider.

PMID: 18637162 [PubMed - as supplied by publisher] PMID: 18637162 [PubMed - som leveres af udgiveren]