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Cell Fractionation

Information on the cell fractionation technique here.

Cell Lysis Methods Table of Contents

• What is Cell Fractionation?
• Definition
• Background
• Applications
• Limitations
• Factors to consider for Cellular Fractionation
• Protocol
• Other Cell Fractionation Protocols
• Troubleshooting Cell Fractionation
• Discussion Topics for Cell Fractionation
• Get Help on your Cell Fractionation Problem

What is Cell Fractionation?

Cell fractionation is the separation of homogeneous sets of compartments of organelles from a population of heterogeneous cells.

Background on Cell Fractionation?

Cell fractionation requires cell lysis, filtration and then centrifugal separation of organelles and subcellular molecules into fractions that can be collected.

Steps in Cell Fractionation

• Disruption (homogenisation) of cells and liberation of organelles.
• Macro Filtration
• Purification of cell components.