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Buffers and Solutions

Commonly used chemicals, solutions, and buffers for the research laboratory. Alphabetically Arranged.

EDTA Stock Solution 0.5 M

0.5 M EDTA Stock Recipe (100 mL) :

• Dissolve 16.81 g of EDTA
• Add distilled water to 90 mL
• Adjust pH to 7.0
• Adjust volume to 100 mL with Water

0.5M EDTA Storage : EDTA is stable at room temperature

PBS (Phosphate Buffered Saline)

PBS Stock Recipe (1 L) :

• Dissolve in 800 mL of Water H20:
• 80 g of NaCl 
• 2 g of KCl
• 26.8 g of Na2HPO4 - 7 H20
•  2.4 g of KH2PO4
• Adjust pH to 7.4 with 1M HCl
• Adjust volume to 1 L with Water

PBS Storage : PBS is stable at room temperature, or can be stored at 4 degrees C.

Uses: PBS is a buffer commonly used to wash cells or western blot membranes.

TBS  (Tris Buffered Saline)

1X TBS Recipe (1 L) :

TBS Storage : TBS is stable at 4 degrees C for 4 months

TBST  (Tris Buffered Saline Tween)

1X TBST Recipe (1 L) : 1 mL of Tween-20 in 1 L of TBS buffer

TBST Storage : TBST is stable at 4 degrees C for 4 months

Uses: TBST is a buffer for washing western blot membranes.

LB Broth   ( Luria Bertani )

LB Broth Recipe (1 L) :

• Dissolve 10 g Bacto-tryptone
• 5 g Bacto-yeast Extract
• 5 grams NaCl
• Adjust volume to 1 L with Water
• Put in Stir Bar and dissolve
• Autoclave

LB Broth : LB Broth is stable at room temperature R/T for several months. If there any signs of contamination or it becomes cloudy, discard and make fresh.