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Buffers and Solutions

Commonly used chemicals, solutions, and buffers for the research laboratory. Alphabetically Arranged.

 

EDTA Stock Solution 0.5 M

0.5 M EDTA Stock Recipe (100 mL) :

0.5M EDTA Storage : EDTA is stable at room temperature

 

PBS (Phosphate Buffered Saline)

PBS Stock Recipe (1 L) :

PBS Storage : PBS is stable at room temperature, or can be stored at 4 degrees C.

Uses: PBS is a buffer commonly used to wash cells or western blot membranes.

 

TBS  (Tris Buffered Saline)

1X TBS Recipe (1 L) :

  • Dissolve 6.05g Tris and 8.76 NaCl in 800 mL of Water.
  • Adjust pH to 7.5 with 1M HCl
  • Adjust volume to 1 L with Water.
  • TBS Storage : TBS is stable at 4 degrees C for 4 months

     

    TBST  (Tris Buffered Saline Tween)

    1X TBST Recipe (1 L) : 1 mL of Tween-20 in 1 L of TBS buffer

    TBST Storage : TBST is stable at 4 degrees C for 4 months

    Uses: TBST is a buffer for washing western blot membranes.

     

     

    LB Broth   ( Luria Bertani )

    LB Broth Recipe (1 L) :

    LB Broth : LB Broth is stable at room temperature R/T for several months. If there any signs of contamination or it becomes cloudy, discard and make fresh.

     


     

     

     



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