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TAE Buffer - Buffer Recipes

Molecular Station Buffer Home. Recipes and protocols for making common research buffers, media, and solutions.

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TAE Buffer

TAE (Tris / acetate / EDTA) buffer.

For 50 X Stock Add:

- 242 g Tris base
- 57.1 ml glacial acetic acid
- 37.2 g Na2EDTAˇ2H2O
- H2O to 1 liter

For Working solution, pH 8.5 Add:

- 40 mM Trisˇacetate
- 2 mM Na2EDTAˇ2H2O

Additional comments for TAE Buffer:




Other Buffer Recipes

For more buffer recipes, see the Buffer Encyclopedia.