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Gene expression is measured in many ways including mRNA and protein expression, however protein expression is one of the best clues of actual gene activitiy since proteins are usually final catalysts of cell activity.
Protein expression can be analyzed using the following methods:
Protein microarrays and high throughput (HT) mass spectrometry (MS) can provide a snapshot of the proteins present in a biological sample. Bioinformatics is very much involved in making sense of protein microarray and HT MS data; the former approach faces similar problems as with microarrays targeted at mRNA, the latter involves the problem of matching large amounts of mass data against predicted masses from protein sequence databases, and the complicated statistical analysis of samples where multiple, but incomplete peptides from each protein are detected.
Discuss and post questions about protein expression in the Bioinformatics Forum.
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